Author(s)

Hai Huang

Affiliation(s)

Department of Critical Care Medicine, Union Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, China.

Corresponding Author

Hai Huang

ABSTRACT

To study the drug resistance and molecular epidemiological characteristics of Acinetobacter baumannii (AB) isolated from patients with intracranial infection (ICI) after neurosurgery. From March 2019 to April 2021, 107 ICI patients and 102 non-ICI patients with AB detected in the cerebrospinal fluid culture of the Neurosurgery Department of Critical Care Medicine, Changjiang Shipping General Hospital were selected as the research objects. The clinical data of the two groups were collected, and the cerebrospinal fluid of the ICI patients was collected. Bacterial culture and drug susceptibility test were carried out, and the molecular epidemiological characteristics of AB were analyzed by pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) techniques. The age and proportion of diabetes in the ICI group were higher than those in the non-ICI group (P <0.05). A total of 107 AB strains were detected in 107 ICI patients, and all of them had different degrees of resistance to commonly used antibacterial drugs. PFGE showed that 107 AB strains were divided into 7 clonotypes, including 91 strains of type A, 6 strains of type B, 4 strains of type C, 2 strains of type D, 2 strains of type E, 1 strain of type F and 1 strain of type G. MLST typing results showed that 107 strains of AB could be divided into 14 ST genotypes, among which the more common genotypes were ST208, ST195, ST75, ST92 and ST1696, and the number of strains were 64, 17, 9, 5 and 3, respectively. All other ST genotypes were 1 strain. Homology analysis by eBUST software showed that there were 97 strains (90.65%) of genotypes ST75, ST92, ST136, ST138, ST195 and ST208, all of which belonged to clonal complex 92 (CC92) and only the gpi allele locus existed The remaining 10 strains belonged to 8 ST genotypes. Patients with ICI after neurosurgery are older and often complicated with diabetes mellitus. The AB flora has certain resistance to the commonly used antibacterial drugs at the present stage, and the results of PFGE and MLST both show the prevalence of dominant clones.

KEYWORDS

Neurosurgery; Intracranial infection; Acinetobacter baumannii; Drug resistance; Epidemiology.

CITE THIS PAPER

Hai Huang. Drug resistance and molecular epidemiology of acinetobacter baumannii isolated from patients with intracranial infection after neurosurgery. World Journal of Biomedical Sciences. 2023, 1(1): 10-15.

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